MCB2010C – Microbiology: Lab Safety Orientation


[MUSIC PLAYING] Microbiology lab
safety orientation. After completing this video,
you should be able to one, demonstrate proper primary
and secondary containment procedures, and two, explain the
procedures involved in dealing with a laboratory emergency. Primary containment. Primary containment concerns
the protection of personnel and the laboratory
environment from exposure to infectious microbes. Proper microbiological
techniques, such as the safe transport
and disposal of cultures along with the correct use
of personal safety equipment, such as gloves
and safety goggles go a long way toward
accomplishing the goal of primary containment. Secondary containment. Secondary containment
deals with protecting the outside environment
from exposure to infectious organisms. It depends principally on
the design of the laboratory and the availability
of equipment, as well, laboratory workers should
maintain the lab’s safety features, such as keeping
a closed door closed or leaving an exhaust fan on. Prior to the lab. Dress appropriately
for the lab– no open toed shoes or
sandals, and avoid clothing with baggy sleeves
that could catch fire or hinder your movement. Know the location of the
eyewash, safety shower, fire extinguisher,
and first aid kit. Take a moment to
learn their operation. During the lab. Always wear a lab coat,
gloves, and safety goggles while working in the lab. The lab coat should
only be used during lab and should remain in the lab. Even discounting
potential biohazards, a lab coat will
protect your clothing. There is a reason many of
the chemicals you will be working with are called stains. Wash your hands prior
to beginning the lab and just before leaving as well. Tie back any long hair, it is
both a source of contamination and a fire hazard. Disinfect your benchtop
with a disinfectant prior to beginning work and just
before leaving the laboratory. Disposal of
contaminated materials. Dispose of plastic petri dishes,
swabs, disposable gloves, inoculating tools, and
similar non-reusable items in the biohazard container. Reusable supplies such as
culture test tubes and glass pipettes should have
all labels removed before being placed in a
rack or container designated for autoclaving. Used microscope slides
should be placed in a container for
autoclaving or soaked in a disinfectant solution
for a minimum of 30 minutes before being discarded. The international biohazard
symbol on the containers not only marks the contents for
autoclaving prior to disposal, but also cautions
anyone in the room as to the possibly hazardous
nature of the items inside the containers. And remember, do not
overfill the container, and never force objects
into the container. Safety considerations. If you are pregnant
or if you feel you shouldn’t be in the lab
because of health concerns, candidly discuss
with your instructor. If your skin is exposed
to microorganisms as a result of a
spill, immediately wash with antiseptic. In the event of a spill, notify
your instructor and your lab partners immediately. Broken glass and
bacterial cultures are a hazardous combination. With your instructor’s
approval, cover the spill with paper towels and saturate
the towels with disinfectant. After 15 to 20 minutes,
carefully wipe up the spill and discard the paper towels
in the biohazard container for autoclaving. Discard the broken glass
in the sharps container. [MUSIC PLAYING]